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Related Experiment Video

Updated: Jan 9, 2026

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

Portable RT-LAMP Platform for Rapid Point-of-Care Detection for SFTSV.

Gi Chan Lee^1,2, Beom Kyu Kim^1,2, Sania Batool¹

¹Department of Microbiology, Chungbuk National University College of Medicine and Medical Research Institute, Cheongju, Chungbuk 28644, Republic of Korea.

Journal of Microbiology and Biotechnology

|December 9, 2025

Summary

A new portable diagnostic tool rapidly detects Severe Fever with Thrombocytopenia Syndrome virus (SFTSV) using Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP). This cost-effective method offers accurate SFTSV detection for improved public health in rural areas.

Keywords:

Dabie bandavirus RT-LAMP SFTSV diagnosis qRT-PCR

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Last Updated: Jan 9, 2026

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Reverse Transcription Loop-Mediated Isothermal Amplification RT-LAMP Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Reverse Transcription Loop-Mediated Isothermal Amplification RT-LAMP Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Published on: May 18, 2020

Area of Science:

Virology
Molecular Diagnostics
Public Health

Background:

Severe Fever with Thrombocytopenia Syndrome virus (SFTSV) is a significant tick-borne threat in East Asia, causing high mortality, especially in the elderly.
Effective disease management necessitates improved diagnostic tools, particularly for resource-limited settings.

Purpose of the Study:

To develop a portable and rapid diagnostic platform for SFTSV detection.
To establish a reliable point-of-care testing (POCT) solution for SFTSV surveillance.

Main Methods:

Designed multiplex Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) primers targeting the SFTSV L gene and β-actin as an internal control.
Developed a portable, clinical-grade device integrating the RT-LAMP assay.
Validated assay sensitivity, specificity, and performance against commercial qRT-PCR kits using clinical specimens.

Main Results:

The RT-LAMP assay achieved a detection limit of 500 RNA copies/reaction, delivering results within 20 minutes.
Demonstrated high specificity with no cross-reactivity with other pathogens.
RT-LAMP platform performance was comparable to commercial qRT-PCR kits.

Conclusions:

The developed portable RT-LAMP platform provides a rapid, accurate, and cost-effective diagnostic tool for SFTSV.
Its ease of use and portability make it suitable for POCT in resource-constrained environments, enhancing SFTSV surveillance and public health outcomes.