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Updated: Apr 30, 2026

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
Published on: June 9, 2014
Equilibrium scaling of phase separated elastin-like polypeptides for engineered condensates
Adam Quintana1, Gabriel P López1, Nick J Carroll1
1Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, NM, USA. ncarroll@unm.edu.
Abstract:
The interaction parameter of the Flory-Huggins free-energy of two phase separating components was experimentally determined and fit to a semi-empirical model. This model captures the temperature and chain length dependence, quantitatively describing phase equilibria and critical temperatures in elastin-like polypeptide systems of VPGVG pentameric repeats. We show temperature driven turbidity measurements of phase separation will recapitulate equilibrium through a simple temperature translation. This integrates both experiment and theory of liquid-liquid phase separation, offering a predictive tool for the ability to engineer ELP condensates and coacervates by tuning number of pentamers, temperature and concentration.
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