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Related Concept Videos

Brain Imaging01:14

Brain Imaging

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Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans),  magnetic resonance imaging (MRI),  functional magnetic resonance imaging (fMRI), and Transcranial Magnetic...
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Second harmonic generation for brain imaging: pathology-related studies.

Mikhail Paveliev1, Anastasiia Melnikova1, Dmitry V Samigullin2,3

  • 1Institute of Physics, Kazan Federal University, 16a Kremlyovskaya St., Kazan 420008, Russia.

Biophysical Reviews
|December 10, 2025
PubMed
Summary

Second harmonic generation (SHG) microscopy offers advanced brain imaging for unstained thick tissues and live subjects. This technique aids in visualizing pathologies like trauma, tumors, and neurodegenerative diseases.

Keywords:
Brain imagingBrain–computer interfaceCollagenGliomaMachine learningSecond harmonic generation

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Area of Science:

  • Neuroscience
  • Biomedical Optics
  • Microscopy

Background:

  • Traditional brain microscopy faces challenges with contrast and imaging thick tissues.
  • Non-linear optical microscopy techniques are needed to overcome these limitations.

Purpose of the Study:

  • To review the state-of-the-art applications of Second Harmonic Generation (SHG) microscopy in brain imaging.
  • To discuss method development and artificial intelligence applications in SHG microscopy for neuroscience.

Main Methods:

  • Second Harmonic Generation (SHG) microscopy utilizes non-linear optical effects for intrinsic contrast.
  • SHG enables imaging of collagen and protein fibrils in unstained thick brain tissues and live subjects.
  • Combined SHG and Third Harmonic Generation (THG) microscopy resolves microstructures like blood vessels and astrocyte endfeet.

Main Results:

  • SHG microscopy provides a powerful tool for visualizing pathological processes including brain trauma, fibrosis, tumorigenesis, and neuroinflammation.
  • It is effective for imaging astrocytes and nerve fiber microtubules.
  • Combined SHG and THG resolve gliovascular interfaces and blood vessel wall microstructure.

Conclusions:

  • SHG microscopy represents a significant advancement for brain imaging, offering high contrast and deep tissue penetration.
  • The technique is crucial for studying various neurological diseases and conditions, including Alzheimer's disease, spinal cord injury, and glioma.
  • Future directions include further method development and integration of artificial intelligence for enhanced data analysis.