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ST-FPM: suppressor tunable FPM for high-quality and highly robust full-field reconstruction.

Jingze Zheng1, Yuncheng Wang1,2, Yiwen Chen1,2

  • 1Key Laboratory of Photoelectronic Imaging Technology and System, School of Optics and Photonics, Beijing Institute of Technology, Beijing 100081, China.

Biomedical Optics Express
|December 10, 2025
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Summary

Fourier ptychographic microscopy (FPM) full-field reconstruction is improved by a new suppressor tunable FPM (ST-FPM) method. This approach enhances image details and robustness, overcoming limitations of existing algorithms for digital pathology applications.

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Area of Science:

  • Microscopy
  • Computational Imaging
  • Digital Pathology

Background:

  • Fourier ptychographic microscopy (FPM) enables high-resolution, large field-of-view imaging.
  • Current FPM reconstruction methods suffer from vignetting artifacts or blurred details due to improper feature utilization.

Purpose of the Study:

  • To develop a suppressor tunable FPM (ST-FPM) method for high-quality, robust full-field reconstruction.
  • To address limitations in existing FPM reconstruction algorithms.

Main Methods:

  • Introduced a weighted term with a tunable suppressor in the loss function's gradient.
  • Modulated the loss in the feature domain to guide reconstruction and improve detail recovery.

Main Results:

  • ST-FPM achieved full-field reconstruction with clear, reliable details and excellent quality.
  • Demonstrated significant advantages in reconstruction details and robustness over existing methods through simulations and experiments.

Conclusions:

  • ST-FPM offers flexible adjustment of feature utilization for diverse samples.
  • Provides a promising direction for advanced full-field reconstruction in microscopy.