Development and Validation of an LC-MS/MS Method for Quantifying Phytohormones Related to Tomato Shelf Life

Area of Science:

• Agricultural Chemistry
• Plant Science
• Analytical Chemistry

Background:

• Phytohormones regulate crucial plant processes like growth and ripening.
• Quantifying these signaling molecules in food is challenging due to low concentrations and complex structures.

Purpose of the Study:

• To develop and validate a sensitive LC-MS/MS method for simultaneous quantification of seven phytohormones in tomato fruit.
• To assess the method's performance regarding extraction, cleanup, and matrix effects.

Main Methods:

• Optimization of extraction, solid-phase extraction cleanup, and mobile phase for high recovery (85-95%).
• Utilized a C18 column for chromatographic separation and triple quadrupole mass spectrometry in MRM mode for detection.
• Method validation according to US-FDA and EC 2021/808 guidelines.

Main Results:

• Achieved high linearity (R² > 0.98), precision, and robustness with low detection limits (down to 0.05 ng/mL).
• Demonstrated reduced matrix effects and high recovery rates.
• Identified significant variations in phytohormone profiles (e.g., abscisic acid, salicylic acid) across tomato samples from different geographic origins.

Conclusions:

• The validated LC-MS/MS method provides a reliable and sensitive platform for phytohormone analysis in tomatoes.
• This approach supports post-harvest quality assessment and food metabolomics research.
• The method is transferable for monitoring bioactive compounds in other food crops.