In-channel cryoprobe extraction for mediastinal and transbronchial cryobiopsy: a novel technique (cross-sectional...

Area of Science:

• Interventional Pulmonology
• Endoscopic Procedures
• Diagnostic Sampling Techniques

Background:

• Cryobiopsy offers high diagnostic yields for lung and mediastinal sampling.
• Conventional cryobiopsy requires bronchoscope and cryoprobe removal, compromising safety and efficiency.
• A novel in-channel cryobiopsy extraction method was developed to maintain continuous visualization and simplify workflow.

Purpose of the Study:

• To evaluate the feasibility, safety, and efficacy of an in-channel cryobiopsy extraction method.
• To compare diagnostic yield, procedure time, and complication rates with traditional cryobiopsy techniques.

Main Methods:

• Prospective study involving 30 mediastinal (C-Cryo-EBUS) and 10 transbronchial (C-TBCB) cryobiopsies using 1.1 and 1.7 mm cryoprobes.
• Tissue samples extracted directly through the bronchoscope's working channel post-freeze without scope removal.
• Assessment of procedural feasibility, diagnostic yield, procedure time, complications, and sample quality.

Main Results:

• 100% diagnostic yield achieved for both mediastinal and transbronchial cryobiopsies.
• No complications or bronchoscopic damage observed.
• Mean procedure times were 15.7 ± 6.3 min (C-Cryo-EBUS) and 14.6 ± 5.8 min (C-TBCB), with excellent sample quality.

Conclusions:

• In-channel cryoprobe extraction is a safe, feasible, and reproducible alternative to conventional cryobiopsy.
• The technique preserves endoscopic visualization, reduces procedure time, and maintains diagnostic sample quality.
• Implementation can optimize interventional pulmonology workflows for safer, more efficient cryobiopsy practices.