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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrum: Interpretation01:24

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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Updated: Jan 9, 2026

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
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Click-and-Analyze: Automated Pinpoint Ambient Mass Spectrometry with Sheath-Flow Probe Electrospray Ionization.

Lei Li1,2, Qiangqiang Xie1, Lee Chuin Chen2

  • 1School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China.

Mass Spectrometry (Tokyo, Japan)
|December 11, 2025
PubMed
Summary
This summary is machine-generated.

A new robotic system with automated positioning enables precise ambient mass spectrometry for analyzing various samples, including food and beverages, with stable results.

Keywords:
pinpoint ambient MSprobe electrospray ionizationrobotic MSsheath flow

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Robotics

Background:

  • Ambient mass spectrometry (AMS) allows for direct analysis of samples without extensive preparation.
  • Precise sample positioning is crucial for reproducible and accurate AMS measurements.
  • Existing AMS systems often lack automated capabilities for targeted sampling.

Purpose of the Study:

  • To develop and demonstrate a robotic sheath-flow probe electrospray ionization mass spectrometry system.
  • To enable pinpoint ambient mass spectrometry with automated sample positioning.
  • To analyze diverse, water-rich samples with high precision and stability.

Main Methods:

  • Integration of a 3-axis Cartesian robot with dual cameras (global and local) for automated probe positioning.
  • Development of a graphical user interface for designating sampling spots via mouse-clicking.
  • Utilization of a microcontroller for precise voltage control, current monitoring, and probe-sample contact detection.
  • Fully automated sampling, transfer, ionization, and cleaning processes controlled by a computer.
  • Stable sheath liquid delivery using a programmable syringe pump.

Main Results:

  • Successful analysis of aqueous standards, beverages, and water-rich soft materials (jelly, fruit slices).
  • Achieved stable ion signals with negligible carry-over between measurements.
  • Demonstrated pinpoint accuracy in ambient mass spectrometry through automated positioning.

Conclusions:

  • The developed robotic AMS system offers enhanced precision and automation for analyzing complex samples.
  • The system's design facilitates targeted analysis of specific locations on various materials.
  • This technology advances the capabilities of ambient mass spectrometry for diverse applications.