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Updated: Jan 9, 2026
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
Published on: May 10, 2022
Ubiquitin-conjugating enzyme E2S serves as a prognostic marker for skin cutaneous melanoma
Guojun Du1, Lei Li1, Yunhui Jiang2
1Emergency Department, Jingmen People's Hospital, Jingchu University of Technology Affiliated Jingmen People's Hospital, Jingmen, China.
Background:
This study investigates the expression of ubiquitin-conjugating enzyme E2S (UBE2S) as a significant prognostic marker for skin cutaneous melanoma (SKCM) and its association with the tumor microenvironment (TME). The research aimed to advance precision oncology by identifying novel therapeutic targets for SKCM.
Methods:
UBE2S expression in normal skin and SKCM tissues was analyzed using Sangerbox, Gene Expression Profiling Interactive Analysis 2 (GEPIA2), and DiSignAtlas databases. Its relationship with SKCM prognosis was examined through GEPIA2, Sangerbox, Ualcan, and TISIDB. The Human Protein Atlas (HPA) database assessed UBE2S protein expression and localization in cancerous and adjacent tissues, normal skin single-cell subgroups, and various cell lines. The CellTracer database explored UBE2S expression and cell differentiation in SKCM single-cell subgroups. CancerSEA and CellTracer databases were utilized to explore UBE2S's role in SKCM single-cell analysis, while TISIDB examined its correlation with the TME.
Results:
Data from various databases showed significantly higher UBE2S messenger RNA (mRNA) and protein levels in cancer tissues compared to normal tissues. UBE2S, mainly found on the cell membrane, is linked to poor patient prognosis (P<0.05). UBE2S was highly expressed in normal skin, SKCM immune cells, and non-immune cell subpopulations, with a strong correlation to immune-related indicators in the SKCM TME (P<0.001). The A-431 and SK-MEL-30 cell lines ranked 7th and 12th in UBE2S expression among all cell lines. UBE2S expression was also positively linked to proliferation, invasion, metastasis, cell cycle, and quiescence (P<0.05).
Conclusions:
UBE2S has the potential to serve as a prognostic biomarker for SKCM, demonstrating a strong correlation with cellular infiltration within the SKCM TME and the functional status of individual cells.
