Single-Cell Multi-Omics Sequencing Technologies and Their Applications in Livestock and Poultry Research

Area of Science:

• Animal Science
• Genomics
• Molecular Biology

Background:

• Conventional bulk sequencing averages signals, obscuring cellular heterogeneity.
• Single-cell multiomics integrates genomics, epigenomics, transcriptomics, and proteomics at the single-cell level.
• This approach overcomes bulk sequencing limitations for high-resolution molecular mapping.

Purpose of the Study:

• To review the development and principles of single-cell multiomics technologies.
• To summarize applications in livestock and poultry research.
• To discuss challenges and future opportunities in the field.

Main Methods:

• Systematic review of single-cell multiomics sequencing technologies.
• Analysis of applications in livestock and poultry research.
• Identification of current challenges and future directions.

Main Results:

• Single-cell multiomics enables detailed resolution of cellular heterogeneity.
• It allows identification of rare, functionally critical cell subpopulations.
• Applications include dissecting biological bases of reproduction, growth, meat production, and disease resistance in livestock and poultry.

Conclusions:

• Single-cell multiomics is a powerful tool for understanding desirable phenotypes in livestock and poultry.
• It offers novel approaches to animal science research.
• The field presents significant opportunities for future advancements.