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Updated: Jan 7, 2026

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression
Published on: March 17, 2020
miR-3613-5p promotes lung cancer progression by targeting and regulating XPO6
Xingya Wang1, Huichao Li2, Xianzhen Wu3
1Department of Respiratory and Critical Care Medicine, Chongqing Southwest Aluminium Hospital, Chongqing, 401326, China.
Background:
MicroRNAs (miRNAs) exert a crucial role in the progression of lung cancer (LC) and can serve as prognostic biomarkers.
Aim:
To analyze the prognostic value of miR-3613-5p and its regulatory effect on XPO6 in LC, this study was conducted.
Methods:
The expression of miR-3613-5p and XPO6 was detected using qRT-PCR. The miRDB database was employed to predict XPO6 as a target gene of miR-3613-5p, and a dual-luciferase reporter assay was further used to verify their interaction. Cell proliferation was assessed using the CCK-8, while cell migration and invasion were evaluated via the Transwell.
Results:
miR-3613-5p was notably overexpressed in LC, and its expression was notably associated with smoking history, TNM stage, tumor size, and lymph node metastasis. High miR-3613-5p expression was closely related to poor survival in patients diagnosed with LC, and miR-3613-5p could act as an independent prognostic factor. Database prediction results indicated that miR-3613-5p directly targets XPO6, which was further verified by the dual-luciferase reporter assay. Additionally, XPO6 could offset the promoting effect of miR-3613-5p.
Conclusion:
The miR-3613-5p-XPO6 axis may act as a LC therapeutic target, and novel therapeutic strategies for LC could be developed based on this axis.
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