[Osteoplastic materials based on three-dimensional PLGA matrices, adenoviral vectors with the BMP2 gene and platelet
I A Nedorubova1,2, V P Basina1,2, A V Mironov1,3
1Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia.
Objective:
Investigate the effect of platelet gel addition on the biological properties of 3D PLGA-based matrices impregnated with adenoviral constructs carrying the BMP2 gene.
Materials And Methods:
The matrices were obtained using antisolvent 3D printing. The release kinetics of adenoviral vectors from matrices were studied by spectrophotometry for the presence of viral DNA. The cytocompatibility of the matrices was assessed using the MTT test and by staining cells with fluorescent dyes. To assess the osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs), the expression of osteogenic marker genes analyzed using real-time PCR and the activity of alkaline phosphatase was determined spectrophotometrically.
Results:
The studied gene-activated matrices based on PLGA and platelet gel demonstrated high cytocompatibility when incubated with MSCs. Viral particles released from the matrices effectively transduced cells and promoted the induction of osteogenic differentiation of MSCs. The inclusion of platelet-rich plasma into the gene-activated matrices enabled prolonged release of adenoviral constructs and a significantly enhanced cell proliferation and osteogenic differentiation of MSCs.
Conclusion:
The use of gene-activated matrices based on PLGA, platelet gel, and adenoviral constructs with the BMP2 gene will accelerate bone regeneration due to the synergistic action of the osteoinductor produced as a result of viral vectors transduction and the components of platelet-rich plasma.
