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[Cytocompatibility of hydroxyapatite coating for titanium implants, applied by radio-frequency magnetron sputtering
V P Basina1, I I Zadiriev2, A V Mironov3
1Research Centre for Medical Genetics, Moscow, Russia.
Objective:
Study of cytocompatibility of hydroxyapatite coating for titanium implants applied by high-frequency magnetron sputtering at low pressures in an inert gas atmosphere.
Materials And Methods:
Hydroxyapatite was deposited on titanium substrates using the HF magnetron sputtering method. The cytocompatibility of the materials was assessed by incubating with multipotent mesenchymal stromal cells (MSCs) using the MTT assay. Adhesion properties were studied by staining cells with fluorescent dyes and using scanning electron microscopy.
Results:
The studied titanium materials possess high cytocompatibility. The number of living cells on the 7th day was 100.0±5.6% and 108.5±8.7% for materials with and without coating, respectively. Furthermore, coating titanium substrates with hydroxyapatite resulted in a statistically significant 1.7±0.4-fold increase in the number of adhered MSCs compared to uncoated materials.
Conclusion:
The hydroxyapatite coating applied using HF magnetron sputtering significantly improves cell adhesion to titanium materials. Thus, the obtained results provide a basis for the development of new implants with functionalized surfaces.
