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Silence of Transmembrane Channel-Like 5 Inhibits Cell Proliferation, Migration, and Invasion While Promoting

Jinnan Wan1, Yang Li1, Zhimin Liu1

  • 1Department of Thyroid and Breast Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Cancer Research and Treatment
|December 11, 2025
PubMed
Abstract

Insights

Silencing Transmembrane Channel-Like 5 (TMC5) inhibits breast cancer cell proliferation, migration, and invasion while promoting apoptosis. Further in vivo studies are needed to explore TMC5 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Transmembrane Channel-Like 5 (TMC5) is implicated in tumor promotion across various cancers.
  • The specific role of TMC5 in breast cancer cell function requires further elucidation.

Purpose of the Study:

  • To investigate the impact of TMC5 silencing on key breast cancer cell behaviors.
  • To assess the effects on proliferation, apoptosis, migration, and invasion in breast cancer cell lines.

Main Methods:

  • Transfection of TMC5-specific small interfering RNA (siTMC5) into MDA-MB-231 and MCF-7 cell lines.
  • Co-culture with 740Y-P and subsequent determination of proliferation, apoptosis, migration, invasion, and p-AKT expression.

Main Results:

  • TMC5 silencing reduced proliferation, migration, and invasion rates while increasing apoptosis in both cell lines.
  • Silencing TMC5 led to decreased p-AKT/AKT expression.
  • Co-culture with 740Y-P reversed these effects, increasing proliferation, migration, and invasion while decreasing apoptosis.

Conclusions:

  • TMC5 silencing demonstrates potential to inhibit breast cancer progression by reducing proliferation, migration, and invasion, and promoting apoptosis.
  • Further in vivo validation is necessary to establish TMC5 as a viable therapeutic target for breast cancer treatment.
Keywords:
AKT pathwayBreast neoplasmsMechanismTransmembrane channel-like 5Tumor progression

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