Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Primary and Secondary Growth in Roots and Shoots03:02

Primary and Secondary Growth in Roots and Shoots

60.0K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.0K
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

402
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
402
Meristems and Plant Growth02:36

Meristems and Plant Growth

49.0K
Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
49.0K
Regulation of Transpiration by Stomata02:04

Regulation of Transpiration by Stomata

30.8K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
30.8K
Osmoregulation in Insects01:47

Osmoregulation in Insects

17.4K
Malpighian tubules are specialized structures found in the digestive systems of many arthropods, including most insects, that handle excretion and osmoregulation. The tubules are typically arranged in pairs and have a convoluted structure that increases their surface area.
17.4K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Assessment of Sample Preparation Workflows and Mass Spectrometry Acquisition Strategies for Bottom-Up Proteomics in Rice Tissues.

Journal of agricultural and food chemistry·2026
Same author

An Aggressive Neoplasm with Mixed Epithelial and Sarcoma-like Features, of Uncertain Primary Origin, Presenting as an Upper Lip Mass: A Case Report.

Journal of clinical medicine·2026
Same author

Dehydration-induced condensation of AGO1 modulates miRNA functionality.

The Plant cell·2026
Same author

Cold storage-induced protein denaturation and functional deterioration of freeze-dried surimi powder.

Food chemistry·2026
Same author

The Paradox in AI Influencer Engagement: A Dual Path to Psychological Need Satisfaction and Frustration.

Behavioral sciences (Basel, Switzerland)·2026
Same author

Comprehensive DIA-MS Proteomics of Root Basal Nodes Elucidates Mechanisms of Salt Tolerance in Rice.

Proteomics·2026
Same journal

Identification of the R2R3-MYB transcription factor family in goji and LbaR2R3-MYB3 mediated wax biosynthesis enhancing powdery mildew resistance in tobacco.

Plant cell reports·2026
Same journal

Diverse molecular mechanisms of in vivo haploid induction in plants: from fertilization interference to genome programming.

Plant cell reports·2026
Same journal

PCIS1 is an essential factor in mitochondrial RNA splicing and complex I biogenesis, with distinct effects in null and downregulated mutants.

Plant cell reports·2026
Same journal

Larix gmelinii UGPase enhances vegetative growth in transgenic poplar through an HYH-mediated positive feedback loop.

Plant cell reports·2026
Same journal

Integrative transcriptional regulatory networks governing cereal root responses to heavy metals and drought.

Plant cell reports·2026
Same journal

Functional characterization of MhbZIP62 in regulating drought tolerance in apple.

Plant cell reports·2026
See all related articles

Related Experiment Video

Updated: Jan 8, 2026

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes
08:04

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

482

Root‑preferential OsPUB36 modulates ROS levels and regulates root development.

Sunok Moon1, Yo-Han Yoo2, Ha Eun Park1

  • 1Graduate School of Green Bio-Science & Crop Biotech Institute, Kyung Hee University, Yongin, 17104, Republic of Korea.

Plant Cell Reports
|December 12, 2025
PubMed
Summary
This summary is machine-generated.

OsPUB36, a root-preferential E3 ubiquitin ligase, influences rice root development by modulating reactive oxygen species (ROS) homeostasis. Overexpression shortens primary roots and elongates root hairs, impacting plant growth.

Keywords:
OsPUB36ROSRootRoot hairs

More Related Videos

A Simple Protocol for Mapping the Plant Root System Architecture Traits
11:09

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Published on: February 10, 2023

3.5K
A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
11:27

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

11.3K

Related Experiment Videos

Last Updated: Jan 8, 2026

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes
08:04

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

482
A Simple Protocol for Mapping the Plant Root System Architecture Traits
11:09

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Published on: February 10, 2023

3.5K
A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
11:27

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

11.3K

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Root development is regulated by reactive oxygen species (ROS) gradients.
  • Class III PUB E3 ubiquitin ligases are involved in plant growth.
  • OsPUB36 is a root-preferential E3 ubiquitin ligase in rice.

Purpose of the Study:

  • To investigate the function of OsPUB36 in rice root development.
  • To understand the role of OsPUB36 in reactive oxygen species homeostasis.
  • To explore the potential functional redundancy within the OsPUB31-OsPUB37 gene subclass.

Main Methods:

  • Phylogenetic meta-analysis to identify gene subclasses.
  • Functional characterization of OsPUB36 through overexpression and mutant analysis.
  • RNA-sequencing and proteomic analysis to identify molecular pathways.
  • Histochemical staining to assess hydrogen peroxide levels.

Main Results:

  • Overexpression of OsPUB36 in rice led to shortened primary roots and elongated root hairs.
  • OsPUB36 is localized to the endoplasmic reticulum (ER).
  • Upregulation of class III peroxidases and increased hydrogen peroxide (H₂O₂) levels were observed in root hairs of OsPUB36 overexpressing plants.
  • Loss-of-function mutants showed no significant phenotype, indicating functional redundancy.

Conclusions:

  • OsPUB36 plays a role in regulating rice root development and reactive oxygen species homeostasis.
  • OsPUB36 modulates root hair elongation and primary root length.
  • Functional redundancy exists among class III PUB E3 ubiquitin ligases in rice.