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Multifocal Optical-Resolution Photoacoustic Microscopy With a Masked Single-Element Transducer.

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    Multifocal optical-resolution photoacoustic microscopy (MOR-PAM) significantly boosts imaging speed by using a diffractive optical element and acoustic mask. This innovation overcomes laser repetition rate limitations for faster cellular imaging.

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    Area of Science:

    • Biomedical Optics
    • Microscopy
    • Acoustic Imaging

    Background:

    • Optical-resolution photoacoustic microscopy (OR-PAM) offers high-resolution, sensitive visualization of cellular optical absorption.
    • Conventional OR-PAM speed is constrained by point-by-point scanning and laser repetition rates.

    Purpose of the Study:

    • To develop a faster OR-PAM technique overcoming current speed limitations.
    • To introduce multifocal optical-resolution photoacoustic microscopy (MOR-PAM) for enhanced imaging velocity.

    Main Methods:

    • Utilized a diffractive optical element (DOE) to create 8 focal spots.
    • Employed a custom-designed encoding acoustic mask to differentiate signals from multiple focal spots.
    • Integrated DOE and acoustic mask with a single-element ultrasonic transducer.

    Main Results:

    • Achieved an 8-fold increase in imaging speed compared to conventional OR-PAM.
    • Demonstrated MOR-PAM with a 266 nm laser at a 10 KHz repetition rate.
    • Provided a cost-effective solution for high-speed OR-PAM.

    Conclusions:

    • MOR-PAM effectively accelerates OR-PAM imaging beyond laser repetition rate limits.
    • The proposed method is versatile and applicable to various OR-PAM setups.
    • Enables new applications requiring rapid, high-resolution photoacoustic imaging.