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Updated: Jan 8, 2026
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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
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In vitro production significantly reduces metabolic differences among bovine embryos.
Denis Laloë1, Julie Gatien2, Camille Dupuy2
1UMR1313 GABI, INRAE, Université Paris-Saclay, AgroParisTech, 78350, Jouy-en-Josas, France.
Metabolomics : Official Journal of the Metabolomic Society
|December 13, 2025
Summary
In vitro embryo production significantly reduces metabolic variability compared to in vivo development. However, in vivo embryo sex influences metabolite changes transiently during early development.
Area of Science:
- Reproductive biology
- Embryology
- Metabolomics
Background:
- In vitro production (IVP) of bovine embryos is common, but quality is inferior to in vivo developed (IVD) embryos.
- Metabolomic analysis of spent culture media can predict embryo potential, but sources of variability are unclear.
Purpose of the Study:
- Compare spent culture media metabolomics between IVP and IVD bovine embryos.
- Analyze variations based on embryo stage, grade, and sex.
Main Methods:
- Analyzed spent culture media from IVP and IVD bovine embryos using 1H-NMR spectroscopy.
- Embryos were cultured individually and characterized by stage, grade, and sex.
Main Results:
- IVP significantly reduced inter-embryo metabolic variability (5-10x lower than IVD).
- Embryo stage and grade contributed minimally (1%) to IVP media variability, but significantly (8-18%) to IVD media variability.
- Embryo sex did not globally affect IVD media composition, but metabolite decrease kinetics differed between sexes.
Conclusions:
- Standardized metabolic profiles of IVP embryos raise questions about biotechnology's impact.
- Metabolic sex differences in IVD embryos are transient during early development.
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