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Capillary Electrophoresis: Applications01:30

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Rapid analysis of ginsenosides in biological samples using an efficient embedded dispersive solid-phase

Meiling Wang1, Xiaona Wang2, Hao Cheng1

  • 1School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China.

Journal of Chromatography. A
|December 14, 2025
PubMed
Summary
This summary is machine-generated.

A novel embedded dispersive solid-phase microextraction (E-DSPE) method rapidly analyzes ginsenosides in biological samples. This efficient, cost-effective technique utilizes lecithin@silica particles for accurate quantification.

Keywords:
Biological samplesEmbedded dispersive solid-phase microextractionEnzymatic conversionGinsenosidesLecithin

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Area of Science:

  • Analytical Chemistry
  • Pharmacognosy
  • Materials Science

Background:

  • Ginsenosides are key bioactive compounds in Panax ginseng.
  • Accurate and rapid analysis of ginsenosides in biological matrices is crucial for research and quality control.
  • Existing methods may be time-consuming or require complex sample preparation.

Purpose of the Study:

  • To develop a new, rapid, and efficient method for analyzing ginsenosides in biological samples.
  • To utilize novel lecithin@silica particles for sample preparation.
  • To validate the method's accuracy, recovery, and matrix effects.

Main Methods:

  • Preparation of lecithin@silica particles by embedding SiO2 with lecithin.
  • Optimization of embedded dispersive solid-phase microextraction (E-DSPE) conditions: particle mass, absorption time, sample pH, and elution solvent.
  • Analysis of four ginsenosides (Rg1, Re, Rb1, Rd) in enzymatic solutions, decoction, and urine samples.

Main Results:

  • Optimized E-DSPE conditions achieved high recovery rates (81.94–116.25%) and low relative standard deviation (<2.91%).
  • The method demonstrated acceptable matrix effects (-11.3% to 18.21%) across different biological samples.
  • Lecithin@silica particles proved effective for ginsenoside extraction.

Conclusions:

  • The developed E-DSPE method offers a rapid, green, cost-effective, and efficient approach for ginsenoside analysis.
  • This technique is suitable for the quantitative determination of ginsenosides in complex biological matrices.
  • The lecithin@silica material shows promise for microextraction applications.