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Updated: Jan 8, 2026

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
Published on: January 31, 2025
High-pressure Methanogenesis Reveals Metabolic Adaptation to Dissolved CO2 Limitation
Taiki Katayama1, Hideyoshi Yoshioka1, Masaru K Nobu2
1Institute for Geo-Resources and Environment, Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST).
Abstract:
This study investigated the effects of elevated hydrostatic pressure on methane production and gene expression in a hydrogenotrophic methanogen isolated from subseafloor sediments at biogenic gas hydrate sites, with a focus on the implications of CO2 availability. Using high-pressure cultivation, the methane production rate decreased by 15% at 25 MPa, while a transcriptomic anal-ysis revealed the marked up-regulation of methyl-coenzyme M reductase and ATP synthase. These results suggest that methanogens compensate for pressure-driven constraints on CO2 utilization by increasing the expression of key methanogenic enzymes, underscoring the overlooked role of CO2 in deep biosphere microbial processes.
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