Sequence-Selective 2'-O-Acetyl Modification of RNA Mediated by Duplex Formation with a Reactive Oligonucleotide Probe
Hirotaka Murase1, Mio Eto1, Jeongsu Lee2
1Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Nishi-ku, Kumamoto 860-0082, Japan.
None:
We designed and synthesized an oligonucleotide acetylating reagent (Ac-probe) that selectively acetylates the 2'-OH groups of RNA upon forming a duplex with the target RNA. The Ac-probe can be readily prepared via a post-synthetic modification method using an oligodeoxynucleotide probe containing 4-thio-dT. During the acetylation reaction, 4-thio-dT is regenerated as the reaction proceeds. Notably, an efficient modification was observed when the complementary base of RNA to 4-thio-dT was cytosine or uracil, indicating the selectivity for the pyrimidine base.
Related Concept Videos
02:36Transfer RNA Synthesis
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
03:21RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
01:20Types of RNA
RNA Performs Diverse...