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Functionalized nanopipettes for pH-gated single cell DNA delivery
Shi-Yu Zheng1, Man-Sha Wu1, Shi-Yi Zhang1
1School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.
Abstract:
Here, we present i-motif-functionalized glass nanopipettes that enable pH-triggered DNA release into single living cells. Acid-induced folding of the i-motif drives complementary strand dissociation through the ∼80 nm tip with minimal invasiveness. This pH-gated nanopipette enables reversible, on-demand single cell DNA delivery via rectification changes, establishing a potential platform for intracellular manipulation.
