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Functionalized nanopipettes for pH-gated single cell DNA delivery.

Shi-Yu Zheng1, Man-Sha Wu1, Shi-Yi Zhang1

  • 1School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.

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Summary

Researchers developed pH-triggered DNA release using i-motif-functionalized glass nanopipettes. This technology allows for precise, on-demand DNA delivery into single living cells with minimal invasiveness.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Intracellular delivery of nucleic acids is crucial for various biological applications.
  • Existing methods often face challenges with efficiency, specificity, and invasiveness.
  • Developing precise tools for single-cell manipulation is an ongoing area of research.

Purpose of the Study:

  • To present novel i-motif-functionalized glass nanopipettes for pH-triggered DNA release.
  • To demonstrate the capability of these nanopipettes for on-demand DNA delivery into single living cells.
  • To establish a potential platform for intracellular manipulation and gene therapy.

Main Methods:

  • Fabrication of glass nanopipettes functionalized with i-motif DNA structures.
  • Utilizing pH changes to induce i-motif folding and subsequent DNA release.
  • Employing microscopy and cell culture techniques to assess delivery efficiency and cellular response.
  • Investigating the mechanism of DNA release through the nanopipette tip.

Main Results:

  • Successful demonstration of pH-triggered DNA release from nanopipettes.
  • Achieved minimal invasiveness during DNA delivery into single living cells.
  • Showcased reversible, on-demand DNA delivery capabilities.
  • Confirmed DNA release through the approximately 80 nm nanopipette tip.

Conclusions:

  • I-motif-functionalized glass nanopipettes offer a promising tool for precise intracellular DNA delivery.
  • The pH-gated mechanism enables controlled and on-demand nucleic acid release.
  • This technology holds potential for advancing single-cell therapies and biological research.