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Published on: November 15, 2017
Enhanced-sensitivity profiling of natural products from TLC plates using a facile graphite-based LA-DART-MS platform.
Xingyu Wang1, Jianxing Lv1, Yilin Chen1
1State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210, China. linnanli@shutcm.edu.cn.
A new graphite-assisted technique boosts laser-assisted ionization thin-layer chromatography direct analysis in real time mass spectrometry (LA-TLC-DART-MS). This method enhances detection of natural compounds in citrus herbs, aiding quality control.
Area of Science:
- Analytical Chemistry
- Natural Product Chemistry
Background:
- Direct analysis in real time mass spectrometry (DART-MS) is a powerful technique for analyzing complex mixtures.
- Improving sensitivity and specificity of DART-MS for natural products remains a challenge.
Purpose of the Study:
- To develop a graphite-assisted technique to enhance laser-assisted ionization TLC direct analysis in real time mass spectrometry (LA-TLC-DART-MS).
- To evaluate the method's effectiveness for analyzing compounds in citrus herbs and assessing sample origins.
Main Methods:
- A graphite-assisted approach was integrated with LA-TLC-DART-MS.
- The enhanced technique was applied to analyze citrus herb samples.
Main Results:
- The graphite-assisted LA-TLC-DART-MS significantly improved signal responses for flavonoids, alkaloids, volatile oils, and organic acids.
- Fourteen compounds were identified in citrus herb samples.
- The method successfully differentiated samples from different geographical origins.
Conclusions:
- Graphite-assisted LA-TLC-DART-MS is a promising technique for sensitive and selective analysis of natural products.
- This method holds potential for effective quality control and origin authentication of natural products like citrus herbs.
