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Noninvasive estimation of superficial layer thickness using multi-channel diffuse correlation spectroscopy.

Saeed Samaei^1,2, Daniel Milej^1,2, Keith St Lawrence^1,2

¹Department of Medical Biophysics, Schulich School of Medicine and Dentistry, Western University, 1151 Richmond St., London, N6A 5C1, Canada.

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|December 15, 2025

Summary

This study presents a new method using three-channel diffuse correlation spectroscopy (DCS) to accurately measure superficial layer thickness and cerebral blood flow, improving noninvasive monitoring.

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Area of Science:

Biomedical Optics
Medical Imaging
Physiological Monitoring

Background:

Diffuse correlation spectroscopy (DCS) offers noninvasive monitoring of cerebral blood flow.
Extracerebral tissue layers can interfere with DCS sensitivity to brain tissue.
Accurate layered-model analysis requires precise superficial layer properties.

Purpose of the Study:

To develop and validate a method for quantifying superficial blood flow dynamics and thickness using three-channel DCS.
To improve the accuracy of cerebral perfusion measurements in the presence of superficial layers.

Main Methods:

Utilized three-channel DCS measurements.
Employed simulations and layered phantom experiments for validation.
Adjusted photon count rates at short source-detector separations.

Main Results:

Demonstrated accurate quantification of superficial-layer blood flow index.
Successfully estimated superficial-layer thickness.
Enabled estimation of the lower-layer blood flow index using DCS data.

Conclusions:

The novel three-channel DCS method effectively quantifies superficial layer properties and underlying cerebral blood flow.
This approach enhances the accuracy and reliability of noninvasive cerebral blood flow monitoring.