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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

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The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
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Mass Spectrometry: Overview01:19

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Related Experiment Video

Updated: Jan 8, 2026

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
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LCMSpector: A simple open-source viewer for targeted hyphenated mass spectrometry analysis.

Mateusz Fido1, Etienne Hoesli2, Elisa Cappio Barazzone3

  • 1Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, Zürich, Switzerland.

Plos Computational Biology
|December 15, 2025
PubMed
Summary
This summary is machine-generated.

LCMSpector simplifies targeted mass spectrometry data analysis for life scientists. This free, user-friendly software enables local processing of complex data, making advanced techniques accessible to non-specialists.

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Area of Science:

  • Life Sciences
  • Analytical Chemistry
  • Biotechnology

Background:

  • Hyphenated mass spectrometry techniques are powerful tools in life sciences.
  • Analyzing complex, multidimensional mass spectrometry data is challenging for non-specialists.

Purpose of the Study:

  • To introduce LCMSpector, a user-friendly graphical application for targeted mass spectrometry data analysis.
  • To provide a free, standalone tool for straightforward local data processing across multiple platforms.

Main Methods:

  • LCMSpector is a standalone graphical user interface application.
  • Users specify mass-to-charge ratios for targeted ions and process data locally.
  • The software supports simultaneous processing of multiple files and real-time graph modification.

Main Results:

  • LCMSpector enables straightforward analysis of targeted mass spectrometry data.
  • The application is versatile and applicable to diverse mass spectrometry datasets from various sources.
  • It facilitates calculation of compound concentrations using calibration standards.

Conclusions:

  • LCMSpector democratizes the analysis of targeted mass spectrometry data.
  • Its user-friendly interface and local processing capabilities benefit non-specialist researchers.
  • The tool enhances the accessibility and application of advanced mass spectrometry in life sciences.