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Plasmonic Metal-Phenolic Network Nanoprobes for Multiplex Dual-Mode Immunophenotyping
Lara González-Cabaleiro1,2, Zhixing Lin3,4, Lorena Vázquez-Iglesias1
1CINBIO, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, Spain.
Nano Letters
|December 16, 2025
Summary
Researchers developed dual-mode nanoprobes for precise cell identification. This metal-phenolic network (MPN) technology enhances multiplexed immunophenotyping accuracy and reduces errors in cell analysis.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Cell Biology
Background:
- Accurate multiplexed immunophenotyping is crucial for dissecting cellular heterogeneity.
- Dual-mode detection strategies, combining surface-enhanced Raman scattering (SERS) and fluorescence, offer enhanced accuracy and cross-validation to minimize false positives.
Purpose of the Study:
- To develop novel dual-mode nanoprobes for precise and scalable immunophenotyping.
- To integrate Raman reporters and fluorescent antibodies within a single probe for simultaneous detection.
Main Methods:
- Fabrication of dual-mode nanoprobes using metal-phenolic networks (MPNs) on hollow plasmonic nanocapsules.
- One-step conjugation of biorecognition elements via metal-phenolic coordination.
- Multiplex detection of epidermal growth factor receptor (EGFR) and CD44 using SERS and flow cytometry.
Main Results:
- Demonstrated successful multiplexed detection of EGFR and CD44 in cultured cells.
- Accurately determined cell population distributions (∼63% HER14, ∼37% HEK-293) using SERS, corroborated by flow cytometry.
- Validated the efficacy of MPN nanoprobes for dual-mode detection.
Conclusions:
- Plasmonic MPN nanoprobes offer a robust and scalable platform for accurate immunophenotyping.
- This dual-mode approach enhances cell analysis and holds potential for targeted cell imaging and diagnostics.
