Related Experiment Video

Updated: Jan 8, 2026

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
09:02

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

Published on: September 22, 2023

2.1K

Genome sequences of human coronavirus NL63 diagnosed in southern France

Houmadi Hikmat1,2, Céline Boschi1,2,3, Sarah Aherfi1,2

  • 1IHU Méditerranée Infection, Marseille, France.

Microbiology Resource Announcements
|December 16, 2025
PubMed

Insights

Researchers sequenced 17 human coronavirus NL63 genomes from France. Most identified strains belonged to subgenotype C2, with one belonging to subgenotype B1, providing insights into coronavirus diversity.

Area of Science:

  • Virology
  • Genomics
  • Infectious Diseases

Background:

  • Human coronavirus NL63 (HCoV-NL63) is a common respiratory pathogen.
  • Understanding the genetic diversity of HCoV-NL63 is crucial for epidemiology and control.

Purpose of the Study:

  • To sequence and characterize human coronavirus NL63 genomes from southern France.
  • To determine the genetic diversity and subgenotypic distribution of HCoV-NL63 in the studied population.

Main Methods:

  • Respiratory samples from patients in southern France were collected for diagnostics.
  • In-house multiplex PCR amplification was employed for viral RNA.
  • Next-generation sequencing using Illumina technology was performed to obtain complete genomes.

Main Results:

  • Seventeen complete human coronavirus NL63 genomes were successfully sequenced.
  • Sixteen genomes were classified under subgenotype C2.
  • One genome was identified as belonging to subgenotype B1.

Conclusions:

  • This study provides valuable genomic data on HCoV-NL63 from France.
  • The findings highlight the predominance of subgenotype C2 in the sampled population.
  • Further genomic surveillance is needed to track coronavirus evolution and spread.
Keywords:
coronavirusgenomegenomic surveillancehuman coronavirus-NL63phylogenomics

More Related Videos

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

9.2K
Unbiased Deep Sequencing of RNA Viruses from Clinical Samples09:36

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

17.6K

Related Concept Videos

Viral Mutations00:36

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.5K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
17.8K