Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

83.3K
Overview
83.3K
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

2.8K
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chronic Cholesterol Exposure Disrupts Macrophage Polarization and Cytokine Secretion in a 3D Microenvironment.

ACS omega·2026
Same author

The COSPAR Panel on Planetary Protection and the COSPAR Policy on Planetary Protection: an overview of governance and activities.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same author

Further updates to the Committee on Space Research Planetary Protection Policy for missions to Icy Worlds.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same author

Machine learning-based label-free macrophage phenotyping in immune-material interactions.

Journal of materials chemistry. B·2025
Same author

Effect of Tyrosine-Containing Self-Assembling β-Sheet Peptides on Macrophage Polarization and Inflammatory Response.

ACS applied materials & interfaces·2025
Same author

Hydrogel-Based Tumor Tissue Microarchitecture Reshapes Dendritic Cell Metabolic Profile and Functions.

Advanced healthcare materials·2025

Related Experiment Video

Updated: Jan 8, 2026

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

5.4K

Engineering Adaptive Immunity in 3D: A Patient-Specific Lymphoid Model Using Stromal Networks and Peripheral Blood

Mei ElGindi1, Shaza Karaman1, Jeremy Teo1,2

  • 1Laboratory for Immuno Bioengineering Research and Applications, Division of Engineering, New York University Abu Dhabi, Abu Dhabi, POBox 129188, UAE.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|December 16, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a 3D model of tertiary lymphoid organs using stem cells. This model mimics immune responses and can be used for vaccine screening and studying autoimmune diseases.

Keywords:
immunoengineeringpersonalized therapystromal‐immune crosstalktertiary lymphoid organs (TLOs)tissue engineering

More Related Videos

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
10:44

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Published on: August 9, 2019

7.6K
Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
10:39

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

8.1K

Related Experiment Videos

Last Updated: Jan 8, 2026

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

5.4K
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
10:44

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Published on: August 9, 2019

7.6K
Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
10:39

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

8.1K

Area of Science:

  • Immunology
  • Stem Cell Biology
  • Biomaterials Engineering

Background:

  • Tertiary lymphoid organs (TLOs) are crucial for local adaptive immunity during chronic inflammation.
  • Recreating TLO complexity in vitro is difficult due to challenges in establishing physiologically relevant stromal-immune interactions.

Purpose of the Study:

  • To engineer a 3D lymphoid tissue model using human adipose-derived stem cells (ADSCs) to mimic TLOs.
  • To investigate the functional diversification of stem cell-derived fibroblastic reticular cell (FRC)-like populations and their role in immune microenvironments.

Main Methods:

  • ADSCs were differentiated into FRC-like populations (FRCP1 and FRCP2) within collagen matrices using TNF-α, LT-α, and IL-4.
  • The model was co-cultured with peripheral blood mononuclear cells (PBMCs) and SARS-CoV-2 S1-primed mature dendritic cells.
  • Transcriptional signatures, surface markers, matrix remodeling, and immune responses (B cell activation, cytokine profiles) were analyzed.

Main Results:

  • Two distinct FRC-like subsets (FRCP1 and FRCP2) were generated, exhibiting characteristics of lymph node stromal cells.
  • The model supported antigen-specific B cell activation and polarized immune responses (Th1 or Th2).
  • FRCP1 promoted B cell support and IgM secretion, while FRCP2 enhanced dendritic cell activation and Th1-type chemokine expression.

Conclusions:

  • The 3D lymphoid tissue model successfully recapitulates key aspects of TLOs, demonstrating functional diversification of stem cell-derived stromal cells.
  • This platform enables the study of stromal-immune crosstalk, antibody generation, and immune microenvironments.
  • The customizable system is valuable for personalized vaccine screening, autoimmune disease modeling, and therapeutic development.