Related Experiment Video
Updated: Jan 8, 2026
11:44
Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
Published on: September 18, 2014
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Between defence and delivery: the DNA sensing response to gene electrotransfer
Tanja Jesenko1,2, Masa Omerzel1,2,3, Loree C Heller4
11Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.
Radiology and Oncology
|December 16, 2025
Summary
Gene electrotransfer (GET) delivers DNA into cells, triggering immune responses. Optimizing GET can enhance therapies by controlling these immune reactions for better treatment outcomes.
Area of Science:
- Biomedical Science
- Molecular Biology
- Immunology
Background:
- Gene therapy utilizes recombinant nucleic acids for treating diseases.
- Gene electrotransfer (GET) is a key non-viral method for gene delivery in clinical trials.
- GET uses electric pulses to permeabilize cell membranes for efficient DNA transfer.
Purpose of the Study:
- To investigate the impact of plasmid DNA delivered via GET on cellular responses.
- To understand how GET-induced DNA sensing influences innate immunity and inflammation.
- To explore the dual role of these responses in therapeutic efficacy.
Main Methods:
- Experimental models including tumor, muscle, and skin.
- Application of short, high-intensity electric pulses for gene electrotransfer.
- Analysis of cellular responses, immune stimulation, and inflammatory signaling.
Main Results:
- GET of plasmid DNA, even non-coding, induces local immune stimulation.
- Delivered DNA activates tissue-specific inflammatory signaling pathways.
- Cellular responses to GET can enhance or impede therapeutic outcomes.
Conclusions:
- Cellular responses to GET have a dual nature, presenting both opportunities and challenges.
- Controlled immune activation can boost antitumor and vaccine efficacy.
- Understanding GET mechanisms allows optimization for desired therapeutic effects or reduced off-target impacts.
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