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Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
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Related Experiment Video

Updated: Jan 8, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Phosphatase PPP1CC Regulates the First Lineage Segregation by GAS5 in Mouse Preimplantation Embryos.

Jianwu Wang¹, Yan Zhang¹, Laijin Wu¹

¹Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin, P. R. China.

Cell Proliferation

|December 17, 2025

Summary

The GAS5-PPP1CC-YAP axis controls early mouse embryo development. This pathway regulates YAP dephosphorylation, driving trophectoderm (TE) cell fate specification during preimplantation development.

Keywords:

hippo signalling pathway mouse preimplantation embryo phosphatase PPP1CC reversible phosphorylation subcortical localisation the first lineage segregation

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Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

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Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins

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Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

Area of Science:

Developmental Biology
Cell Biology
Genetics

Background:

YAP, a transcriptional effector of the Hippo pathway, is crucial for lineage specification in mouse preimplantation embryos.
The precise mechanism of YAP dephosphorylation in trophectoderm (TE) versus inner cell mass (ICM) remains unclear.

Purpose of the Study:

To elucidate the mechanism regulating YAP dephosphorylation and its role in TE lineage specification.
To identify key molecules involved in spatial regulation of YAP activity during early embryogenesis.

Main Methods:

Investigated the localization and function of serine/threonine phosphatase PPP1CC.
Examined the interaction between PPP1CC and long non-coding RNA GAS5.
Utilized gene knockdown and overexpression in mouse embryos to assess developmental outcomes.

Main Results:

PPP1CC localizes to TE during blastocyst stage, mediating YAP dephosphorylation and nuclear translocation.
The long non-coding RNA GAS5 facilitates PPP1CC localization to TE, regulating YAP dephosphorylation.
GAS5 knockdown phenocopies PPP1CC deficiency, leading to developmental arrest; GAS5 overexpression biases cells towards TE fate.

Conclusions:

The GAS5-PPP1CC-YAP axis is a critical regulator of first lineage specification in mouse preimplantation development.
This axis highlights the importance of spatially regulated reversible phosphorylation in early embryogenesis.