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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Published on: February 28, 2015

Programmable CRISPR-mediated gold nanoparticle adhesion for visual colorimetric detection.

Kurt T Schalper¹, Rui Yang¹, Xin Guan¹

¹Department of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06032, United States; Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut, 06269, United States.

Biosensors & Bioelectronics

|December 17, 2025

Summary

This summary is machine-generated.

Researchers developed a new CRISPR-based method using gold nanoparticles for sensitive human papillomavirus (HPV) DNA detection. This simple, visual assay offers a promising low-cost solution for point-of-care diagnostics, even in resource-limited settings.

Keywords:

CRISPR-Cas12a diagnostics Colorimetric detection Gold nanoparticles Programmable surface adhesion Stimuli-responsive surface chemistry

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Area of Science:

Nanotechnology
Molecular Biology
Diagnostics

Background:

Gold nanoparticles (AuNPs) aggregation is well-studied for biosensing and nanomedicine.
Programmable surface adhesion of AuNPs using tunable surface chemistry is an underexplored area.

Purpose of the Study:

To introduce a programmable CRISPR-mediated hydrophobic adhesion phenomenon for nucleic acid detection.
To develop a simple, visual-readout colorimetric assay for ultrasensitive DNA detection.

Main Methods:

Functionalization of streptavidin-coated AuNPs with Cy5-ssDNA-biotin probes.
Utilizing CRISPR-Cas12a-mediated ssDNA cleavage coupled with AuNP hydrophobic adhesion.
Integration with recombinase polymerase amplification for enhanced sensitivity.

Main Results:

Demonstrated a novel hydrophobic adhesion mechanism for AuNPs triggered by Cy5 moieties.
Achieved ultrasensitive detection of human papillomavirus (HPV) DNA down to 10 aM.
Validated clinical utility by detecting HPV DNA in cervical swab samples.

Conclusions:

The developed platform offers a new modality for CRISPR-based colorimetric diagnostics.
This method provides a simple, low-cost, and sensitive solution for point-of-care diagnostics, particularly in resource-limited settings.
The surface adhesion mechanism represents a distinct signal transduction pathway compared to conventional aggregation-dispersion systems.