Related Experiment Video
Updated: Jan 8, 2026
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid
Published on: April 11, 2019
CD34 Positive Selection of Cryopreserved Stem Cell Concentrates with the CliniMACS Prodigy Platform and the Tubing
Claudia Bernecker1, Konrad Rosskopf1, Claudia Url1
1Department of Blood Group Serology and Transfusion Medicine, Medical University of Graz, Graz, Austria.
Introduction:
Immuno-magnetic CD34 positive (CD34+) selection is generally used for stem cell boosts after allogeneic stem cell transplantation. In some cases, only cryopreserved cells are available as starting material. We present a new automatic device and technique for preparing and subsequently magnetically enriching these cells.
Methods:
We used the CliniMACS Prodigy® platform in combination with the TS 320 tubing set, equipped with a large 800 mL chamber, which enables to include the DMSO wash, platelet wash, antibody incubation, and antibody wash and the subsequent CD34 enrichment on an automatic basis with only short hands-on time. We performed three validation runs using DNase and sodium citrate for cell preparation to reduce the risk of aggregates and clots.
Results:
Our robust preclinical results show the feasibility and safety of the process with a mean of 53% CD34 yield after thawing and CD34 purity and viability of 93 and 97%, respectively. CD3 log depletion exceeds 5.0 in all 3 cases, which provides optimal GvHD prevention.
Conclusion:
This shows that the updated technique gains unaffected CD34+ cells of high quality, not only from fresh but also from cryopreserved stem cell products.
