OpenIDS2: A low-cost, 3D-printed, open-source platform for reproducible construction of DNA microarray synthesizers

Area of Science:

• Biotechnology
• Synthetic Biology
• Molecular Diagnostics

Background:

• Oligonucleotide synthesis is crucial for life science applications.
• Existing DNA synthesizers can be expensive and complex.
• There is a need for accessible, low-cost synthesis platforms.

Purpose of the Study:

• To present OpenIDS2, a second-generation, open-source inkjet-based DNA synthesizer.
• To improve upon the original OpenIDS platform with enhanced features and accessibility.
• To lower the barrier to entry for laboratory automation in oligonucleotide synthesis.

Main Methods:

• Developed OpenIDS2, a compact (one-third volume) DNA synthesizer.
• Integrated control electronics via a custom PCB.
• Implemented a peristaltic pump system for bulk solution delivery.
• Designed most mechanical components for 3D printing.
• Utilized phosphoramidite chemistry for synthesis.
• Synthesized 15-mer poly(dT) sequences on CPG substrates.

Main Results:

• OpenIDS2 demonstrates reduced volume and improved operational stability.
• 3D-printable components enhance fabrication accessibility and reduce cost.
• The system successfully synthesized and validated 15-mer poly(dT) sequences.
• Urea-PAGE and HPLC confirmed the synthesized DNA sequences.

Conclusions:

• OpenIDS2 provides a practical, open-source, benchtop oligonucleotide synthesizer.
• The platform enhances reproducibility and global accessibility.
• OpenIDS2 serves as an extensible foundation for laboratory automation.