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Updated: Jan 8, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Published on: September 10, 2017
Research advances in capillary electrophoresis coating technology for protein analysis
Mengyuan Xu1, Wei Pan1, Yichen Tian1
1College of Chemistry and Chemical Engineering, College of Life Sciences, Institute of Biomedical Materials and Engineering, Qingdao University, Qingdao, 266071, China.
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Capillary electrophoresis (CE) is primarily a technique for separating and analyzing samples based on their differing physicochemical properties. CE is renowned for its rapid analytical capability and minimal sample requirements, rendering it widely applicable in the biomedical field. However, the silica hydroxyl groups (Si-OH) present on the capillary wall can adversely affect protein separation. Modifying capillary walls through physical adsorption or chemical bonding can suppress protein adsorption, thereby enhancing separation efficiency. This review examines the causes and solutions for protein adsorption, types of capillary coatings, and the application of coated capillaries in protein separation. Finally, it outlines future trends for coated capillaries in protein separation.
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