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Updated: Jan 8, 2026
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
Protocol for isolating and assessing immunomodulatory ability of explant-cultured human amniotic membrane mesenchymal
Srishti Dutta Gupta1, Pritam Banerjee1, Tania Haoladar1
1Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, Nadia, West Bengal 741246, India.
Abstract:
Mesenchymal stem cells (MSCs) are promising for regenerative medicine and cell-based therapies due to their multipotent and immunomodulatory properties. Here, we present a protocol for isolating MSCs from the amnion of the human amniotic membrane (AM-MSCs) using the explant culture approach and their subsequent characterization. We also describe steps for evaluating the immunomodulatory potential of the conditioned medium (CM) harvested from AM-MSCs, enabling assessment of its secretome without direct cell-to-cell contact.
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