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EasyKASP: a simple and fast tool for KASP primer design.

Jian Zhang^1,2, Jingjing Yang^1,3, Changlong Wen^4,5

¹Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

BMC Bioinformatics

|December 19, 2025

Summary

EasyKASP is a new, user-friendly software tool for designing Kompetitive Allele-Specific PCR (KASP) primers for both single nucleotide polymorphisms (SNPs) and insertion-deletions (InDels). This rapid tool demonstrates broad applicability in KASP genotyping studies.

Keywords:

Excel VBA InDel KASP primer design SNP

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Area of Science:

Molecular Biology
Genetics
Bioinformatics

Background:

Kompetitive Allele-Specific PCR (KASP) is a widely adopted, cost-effective genotyping technology for detecting SNPs and InDels.
A significant limitation exists in the availability of automated software for KASP primer design, particularly for InDel variations.

Purpose of the Study:

To develop a user-friendly and automated software tool for designing KASP primers.
To address the scarcity of accessible tools for KASP primer design, especially for InDels.

Main Methods:

Analysis of KASP primer sequence characteristics.
Development of EasyKASP, a software program on the Excel VBA platform.
Validation of EasyKASP-designed primers using KASP technology.

Main Results:

EasyKASP was developed for automated KASP primer design for both SNP and InDel variations.
The software exhibits a rapid average processing time of 0.03 seconds per primer pair.
All 80 SNP and 6 InDel loci validated using EasyKASP primers were successfully amplified and genotyped.

Conclusions:

EasyKASP provides a simple, rapid, and effective solution for KASP primer design.
The tool shows significant potential for broad application in various KASP genotyping studies.