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Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
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An efficient transient expression system for study of endosperm development in plants.

Ruiqiu Fang1, Guihua Lv1, Jianjian Chen1

  • 1Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, Zhejiang, 322100, China.

Plant Physiology and Biochemistry : PPB
|December 20, 2025
PubMed
Summary

Researchers developed a fast Agrobacterium-mediated transient expression system for sweet corn endosperm research. This tool enables efficient gene expression and functional validation, advancing endosperm functional genomics.

Keywords:
AgrobacteriumEndospermKernelSweet cornTransient expression

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • The endosperm is crucial for human nutrition, supplying over 60% of global needs.
  • Endosperm development is a complex, precisely regulated process.
  • Lack of efficient tools for functional validation hinders endosperm research.

Purpose of the Study:

  • To establish a simple, rapid Agrobacterium-mediated transient expression system for endosperm research.
  • To optimize factors influencing transient expression efficiency in sweet corn.
  • To develop a method for efficient gene functional validation in endosperm.

Main Methods:

  • Utilized sweet corn (cultivar 7401) kernels for Agrobacterium infiltration.
  • Systematically examined factors affecting transient expression.
  • Employed Agrobacterium strain GV3101 with helper plasmids for enhanced transformation.
  • Developed a phenotype index for gene function identification.

Main Results:

  • Achieved high transient expression efficiency (up to 100%) using optimized methods.
  • Detected reporter protein eGFP as early as 3 days post-infiltration (DAI).
  • Demonstrated stable expression of reporter proteins up to 10 DAI.
  • Successfully identified gene functional effects using the phenotype index.

Conclusions:

  • Introduced a valuable, efficient tool for endosperm functional genomics research.
  • The system facilitates sustained and efficient gene expression in sweet corn endosperm.
  • Enables large-scale functional genomics studies crucial for understanding endosperm development and improving crop nutrition.