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Smartphone-enabled magneto-immunofluorescence platform for rapid isomiroestrol detection in plants
Nopphakon Phongsanam1, Worapol Sae-Foo2, Samuch Phetduang1
1Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
Talanta
|December 20, 2025
Summary
A new portable magneto-immunofluorescence platform enables rapid isomiroestrol (IME) detection in plants. This cost-effective method uses magnetic nanoparticles and smartphone detection, offering a field-deployable alternative to traditional lab-based assays.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Plant Science
Background:
- Traditional Enzyme-Linked Immunosorbent Assay (ELISA) for isomiroestrol (IME) detection is limited by complex laboratory requirements, extensive washing, and blocking steps.
- There is a need for rapid, cost-effective, and field-deployable methods for IME detection in plant samples.
Purpose of the Study:
- To develop a portable magneto-immunofluorescence platform for sensitive and rapid detection of isomiroestrol (IME) in plant samples.
- To overcome the limitations of conventional laboratory-based analytical techniques for IME quantification.
Main Methods:
- Utilized amino-functionalized magnetic nanoparticles for selective IME capture via a formaldehyde-mediated Mannich reaction.
- Employed a dual-antibody detection system involving monoclonal antibodies and a horseradish peroxidase-labeled polyclonal antibody (pAb-HRP).
- Developed an immunofluorescence detection principle based on the enzymatic conversion of o-phenylenediamine (OPD) by HRP to fluorescent 2,3-diaminophenazine (DAP), measured by a smartphone-based portable device.
Main Results:
- The platform demonstrated excellent linearity (R² = 0.9960) over the concentration range of 0.025-1.5 μg mL⁻¹, with a low detection limit of 0.021 μg mL⁻¹.
- The assay required only two washing steps at ambient temperature, significantly reducing analysis time compared to traditional methods.
- Analytical performance was comparable to standard ELISA techniques, indicating high reliability.
Conclusions:
- The developed portable magneto-immunofluorescence platform offers an accessible, rapid, and reliable solution for field-based IME detection in plant samples.
- This technology provides a cost-effective alternative to conventional spectrofluorometers and complex laboratory equipment.
- The platform facilitates on-site analysis, enabling quicker decision-making in agricultural and research settings.
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