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CRISPR as a next-generation environmental biosurveillance tool for air, land, and water
Benjamín Durán-Vinet1, Jo-Ann L Stanton2, Gert-Jan Jeunen3
1Department of Anatomy, Faculty of Biomedical Sciences, University of Otago, Dunedin 9016, New Zealand; Department of Biochemistry, Faculty of Biomedical Sciences, University of Otago, Dunedin 9016, New Zealand; Department of Immunology and Microbiology, Faculty of Biomedical Sciences, University of Otago, Dunedin 9016, New Zealand; CRISPR hub, Genetics Otago Centre, University of Otago, Dunedin 9016, New Zealand.
Abstract:
Clustered regularly interspaced short palindromic repeats (CRISPR)-based environmental biosurveillance (CRISPR-eBx) offers a portable, specific, sensitive, and cost-effective platform for detecting organisms from environmental nucleic acids. Applications are broad, ranging from pathogen detection to monitoring invasive and endangered species across a range of environmental sources, including water, soil, and air. However, if CRISPR-eBx is to be deployed for novel biological/gene targets and environmental sources, key challenges must be addressed. This review synthesizes recent developments at the intersection of CRISPR technology, computational science, synthetic biology, and biosurveillance. We highlight promising innovations and identify knowledge gaps to present a strategic road map for establishing CRISPR-eBx as a next-generation, frontline biosurveillance solution.
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