Related Experiment Video
Updated: Jan 8, 2026

Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays
Published on: September 20, 2024
Anti-interferon-γ Autoantibodies and Indeterminate QuantiFERONⓇ-TB Gold Plus Assays
Serena Lee1, Annalie J Harris1, Christa S Zerbe1
1Laboratory of Clinical Immunology and Microbiology, NIAID, NIH, Bethesda, Maryland, USA.
None:
Among 852 samples sent for QuantiFERON®-TB Gold Plus Assay (QFN) analysis, between 6% and 11% had IFNγ binding activity, but only 1.2% of indeterminate samples had IFNγ blocking activity. No TB negative samples had blocking activity to IFNγ. Indeterminate QFN results may indicate the presence of blocking anti-IFNγ autoantibodies.
More Related Videos
10:00High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
08:26Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018