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Updated: Jan 8, 2026

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Published on: November 17, 2023

Laser-Activated Microfluidic SERS Substrates.

Milena S Shestopalova^1,2, Denis S Korzhov^1,2, Konstantin N Afanasyev³

¹Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.

|December 22, 2025

Summary

This summary is machine-generated.

This study introduces stable, activatable surface-enhanced Raman spectroscopy (SERS) substrates for microfluidic chips. These substrates enable ultrasensitive detection of extracellular vesicles, crucial for identifying disease biomarkers.

Keywords:

Kelvin probe force microscopy atomic force microscopy extracellular vesicles lab-on-chip technology limit of detection plasmonic nanostructures surface-enhanced Raman spectroscopy

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Area of Science:

Analytical Chemistry
Biotechnology
Materials Science

Background:

Automated microfluidic systems coupled with surface-enhanced Raman spectroscopy (SERS) show promise for ultrasensitive biomaterial detection.
Extracellular vesicles are key targets for disease biomarker discovery (microRNA, mRNA, proteins).
Existing SERS substrates, especially silver-based ones, suffer from instability due to oxidation and contamination, limiting practical use.

Purpose of the Study:

To develop a novel fabrication method and operational concept for stable, activatable SERS substrates.
To enable integration of SERS substrates into automated microfluidic systems for enhanced disease biomarker analysis.
To overcome the limitations of temporal instability in current SERS detection systems.

Main Methods:

Fabrication of SERS substrates designed for immediate activation prior to experimentation.
Integration of nonactivated SERS substrates into microfluidic chips for long-term storage without functional loss.
Development of a theoretical model to assess local electromagnetic field enhancement.
Experimental determination of substrate sensitivity for extracellular vesicle detection.

Main Results:

Demonstrated a method for creating SERS substrates with extended shelf-life and on-demand activation.
Validated the integration of these substrates into chip-based spectral recording chambers for automated systems.
Experimental results confirmed the high sensitivity, enabling rapid detection of individual extracellular vesicles and their clusters from HEK293T cells.

Conclusions:

The proposed SERS substrates offer a stable and practical solution for ultrasensitive extracellular vesicle detection in microfluidic systems.
This advancement facilitates the development of automated platforms for disease biomarker analysis.
The findings pave the way for more reliable and accessible diagnostic tools based on SERS technology.