Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

100
Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a...
100

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plant Hydathodes Detect Microbial Patterns to Close Hydathode Pores and Restrict Leaf Entry.

Phytopathology·2026
Same author

Dissecting morphological changes during floral abscission in <i>Arabidopsis thaliana</i>.

AoB PLANTS·2026
Same author

Discovery of Fusadapamides, Accessory Chromosome-Associated Metabolites Incorporating l-2,3-Diaminopropionic Acid in <i>Fusarium poae</i>.

Journal of natural products·2025
Same author

Alanine aminotransferase contributes to hypoxia sensitivity and dormancy in barley seeds.

The plant genome·2025
Same author

Transcriptional Profiling and Genetic Mapping of Barley Responses to Bacterial Flagellin.

Molecular plant-microbe interactions : MPMI·2025
Same author

More than a passive barrier: algal cell walls play an active role in determining cell shape, cell size, and organelle morphology.

Journal of experimental botany·2025
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

A Tandem Liquid Chromatography&#8211;Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

10.5K

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format.

Whynn Bosnich1, Natalie Hoffmann2, Siddharthan Lakshmanan3

  • 1Ottawa Research and Development Centre, Agriculture and Agri-Food Canada.

Journal of Visualized Experiments : Jove
|December 22, 2025
PubMed
Summary
This summary is machine-generated.

This study presents a method to measure plant stress responses to microbial compounds by assessing ion leakage, peroxidase activity, and callose production. This approach aids in understanding plant-microbe interactions and identifying stress-modulating compounds.

More Related Videos

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
06:43

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

9.1K
Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format
05:51

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Published on: December 5, 2025

299

Related Experiment Videos

Last Updated: May 5, 2026

A Tandem Liquid Chromatography&#8211;Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

10.5K
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
06:43

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

9.1K
Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format
05:51

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Published on: December 5, 2025

299

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Biochemistry

Background:

  • Microbial secondary metabolites trigger plant stress responses during infection.
  • Understanding these plant-microbe interactions is crucial for agriculture and bioprotection.

Purpose of the Study:

  • To develop a method for simultaneously measuring ion leakage, peroxidase activity, and callose production in plant leaf disks.
  • To enable comparative analysis of plant responses to various microbial metabolites and genotypes.

Main Methods:

  • Vacuum infiltration of Arabidopsis or barley leaf disks in a 96-well plate.
  • Measurement of conductivity (ion leakage) after 4-6 hours.
  • Assay of peroxidase activity and callose deposition at 24 hours.

Main Results:

  • The flg22 peptide reliably induces all three measured stress responses.
  • Specific microbial lipopeptides (surfactin, gramillin) and phytotoxins (T-2 trichothecene) elicit distinct response patterns.
  • The method allows for differential responses to be observed across various treatments.

Conclusions:

  • This integrated approach provides a robust platform for studying plant stress signaling pathways.
  • It facilitates chemical genetics and bioprotection research by identifying novel stress-modulating compounds.
  • The method is applicable to plant genetics for mapping and understanding plant-microbe interactions.