Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

38.2K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.2K
Polarity of the Cytoskeleton01:18

Polarity of the Cytoskeleton

24.2K
The intrinsic polarity of cells can be primarily attributed to two factors- i) the asymmetric accumulation of mobile components such are regulatory molecules and subcellular components across the cell and ii) the orientation of polar cytoskeletal filaments that make up the cytoskeletal networks, specifically microfilaments, and microtubules arranged along the axis of polarity. Interactions between the cytoskeletal filaments are crucial for the establishment and maintenance of the polar nature...
24.2K
Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

62.9K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
62.9K
Bond Polarity, Dipole Moment, and Percent Ionic Character02:48

Bond Polarity, Dipole Moment, and Percent Ionic Character

34.5K
Bond Polarity
34.5K
Anionic Chain-Growth Polymerization: Overview01:20

Anionic Chain-Growth Polymerization: Overview

2.5K
The polymerization process that involves carbanion as an intermediate is called anionic polymerization. It is also a type of addition or chain-growth polymerization. Anionic polymerization gets initiated by a strong nucleophile such as an organolithium or a Grignard reagent. The most commonly used initiator for anionic polymerization is butyl lithium. Monomers involved in anionic polymerization must possess a vinyl group bonded to one or two electron-withdrawing groups. For instance,...
2.5K
What are Proteins?01:55

What are Proteins?

236.5K
Overview
236.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How the Extent of Protein Folding and Oligomerization Modulate Condensate Formation and Properties.

The journal of physical chemistry letters·2025
Same author

RNA binding and coacervation promote preservation of peptide form and function across the heterochiral-homochiral divide.

Protein science : a publication of the Protein Society·2025
Same author

Intersegment Transfer and the Dynamical Architecture of Fis Protein-DNA Multimer Complexes.

Journal of the American Chemical Society·2025
Same author

Competition between Nucleic Acids and Intrinsically Disordered Regions within Proteins.

Accounts of chemical research·2025
Same author

Theoretical Understanding of Target Search Dynamics in Horizontal Gene Transfer in Bacteria.

The journal of physical chemistry. B·2025
Same author

A pH-Dependent Coarse-Grained Model for Disordered Proteins: Histidine Interactions Modulate Conformational Ensembles.

The journal of physical chemistry letters·2024

Related Experiment Video

Updated: Jan 8, 2026

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.4K

Various Ways to Be Negative: Biophysical Characterization of Polyanionic Biomolecules.

Noa Binnes1, Ilan Edelstein1, Yaakov Levy1

  • 1Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel.

The Journal of Physical Chemistry. B
|December 23, 2025
PubMed
Summary

Negatively charged biopolymers, called polyanions, have diverse structures and properties. Their unique features allow for various biological functions, explaining their prevalence in life.

More Related Videos

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
09:37

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

44.7K
A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.4K

Related Experiment Videos

Last Updated: Jan 8, 2026

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.4K
Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
09:37

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

44.7K
A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.4K

Area of Science:

  • Biochemistry
  • Biophysics
  • Molecular Biology

Background:

  • Negatively charged biopolymers (polyanions) are essential in biological systems.
  • Their diverse structures raise questions about how biophysical properties relate to function.

Purpose of the Study:

  • Investigate the relationship between polyanion intrinsic features and their biophysical properties.
  • Understand how these properties influence solvent organization and conformational preferences.

Main Methods:

  • All-atom molecular dynamics simulations were used.
  • Eleven representative polyanions (polynucleotides, polypeptides, polysaccharides) were modeled.
  • Simulations were conducted in mono- and divalent counterion environments.

Main Results:

  • Polyanions exhibit significant variations in compactness and flexibility.
  • Conformational preferences are system-specifically modulated by cation identity (e.g., sodium, calcium).
  • Each macromolecular family occupies a distinct region in conformational space.

Conclusions:

  • Polyanions possess tunable biophysical properties exploitable for specific biological functions.
  • Subtle differences in intrinsic features lead to unique properties even within the same family.
  • The biological preference for polyanions over positively charged polymers remains an open question.