Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

7.5K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
7.5K
Treatment for Pulmonary Arterial Hypertension: Receptor Tyrosine Kinase Inhibitors and Calcium Channel Blockers01:26

Treatment for Pulmonary Arterial Hypertension: Receptor Tyrosine Kinase Inhibitors and Calcium Channel Blockers

411
Receptor tyrosine kinase inhibitors (TKIs) and calcium channel blockers (CCBs) are two critical categories of drugs employed in the treatment of pulmonary artery hypertension (PAH). PAH is a disease that causes high blood pressure in the pulmonary arteries, resulting in chest pain, fatigue, and shortness of breath.
TKIs, such as imatinib (Gleevec), are particularly effective in tackling the growth and mitogenic factors that become upregulated in PAH patients. These factors contribute to the...
411

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prefrontal parvalbumin neurons mediate working memory in a task demand-dependent manner.

Nature communications·2026
Same author

A dual role of hippocampal perineuronal nets in plasticity and protection is revealed by improvement and impairment of diet-induced memory dysfunction.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026
Same author

Systemic Piezo1 activation improves cerebrovascular function in Alzheimer's disease.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

VasoTracker 2: Open-source software and hardware for tracking blood vessel diameter and assessing vascular function.

The Journal of physiology·2025
Same author

Brain Capillary Ion Channels: Physiology and Channelopathies.

Physiology (Bethesda, Md.)·2025
Same author

Angiotensin AT<sub>1</sub>R expressing cells within the ventral hypothalamus modulate integrative control of cardiometabolic functions.

iScience·2025

Related Experiment Video

Updated: Jan 7, 2026

One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

25.3K

PIP2 corrects an endothelial Piezo1 channelopathy.

Ahmed M Hashad1, Mohammad M Abd-Alhaseeb1, Xin Rui Lim1

  • 1Department of Pharmacology, Larner College of Medicine, Vermont Center for Cardiovascular and Brain Health, University of Vermont, Burlington, VT 05405.

Proceedings of the National Academy of Sciences of the United States of America
|December 23, 2025
PubMed
Summary

G protein-coupled receptor (GPCR) activation enhances Piezo1 channel activity in brain capillaries. This crosstalk, regulated by phosphatidylinositol-4,5-bisphosphate (PIP2), impacts neurovascular coupling and cerebral blood flow.

Keywords:
GqPCRPIP2Piezo1cerebral blood flowneurovascular coupling

More Related Videos

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

10.1K
Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption
09:20

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Published on: October 4, 2019

5.9K

Related Experiment Videos

Last Updated: Jan 7, 2026

One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

25.3K
Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

10.1K
Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption
09:20

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Published on: October 4, 2019

5.9K

Area of Science:

  • Neuroscience
  • Vascular Biology
  • Cell Physiology

Background:

  • Brain capillaries, specifically endothelial cells (ECs), act as sensors of neural activity, regulating cerebral blood flow through functional hyperemia.
  • Functional hyperemia involves Gαq protein-coupled receptor (GqPCR) activation and mechanosensitive Piezo1 signaling, but the interplay between them is unknown.

Purpose of the Study:

  • To investigate the influence of GqPCR activation on Piezo1 mechanosensitive signaling in brain capillary ECs.
  • To elucidate the molecular mechanisms underlying this crosstalk and its implications for neurovascular coupling.

Main Methods:

  • Patch-clamp electrophysiology on freshly isolated brain capillary ECs.
  • Pharmacological manipulation of GqPCR and downstream signaling pathways.
  • Assessment of Piezo1 activity in ECs from disease models and in vivo functional hyperemia studies.

Main Results:

  • GqPCR activation by prostanoids or muscarinic agonists potentiates Piezo1 channel activity.
  • This potentiation involves Gαq, phospholipase C, and phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis; exogenous PIP2 suppresses Piezo1 activity.
  • ECs from Alzheimer's disease and cerebral small vessel disease models show elevated Piezo1 activity, correctable by PIP2.

Conclusions:

  • GqPCR signaling crosstalks with and enhances Piezo1 activity in brain capillary ECs, mediated by PIP2 levels.
  • Dysregulated Piezo1 activity in disease states can be modulated by PIP2.
  • Findings offer insights into Piezo1 regulation, neurovascular coupling, and potential therapeutic strategies for cerebral blood flow disorders.