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Bioactive potential of Rhizophora apiculata: phytochemical profiling, antibacterial activity, and toxicity evaluation
Krishnamoorthy Santhosh1, Ragul Gunasekaran1, Kamala Kannan2,3
1Marine Biomedical Research Lab and Environmental Toxicology Unit Cellular and Molecular Research Centre, Saveetha Dental College and Hospitals, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.
Abstract:
Rhizophora apiculata, a mangrove plant, is recognised as a rich source of bioactive compounds with significant medicinal and pharmacological potential. This study aimed to investigate the phytochemical composition, antibacterial activity, zebrafish toxicity and promising bioactive constituents of R. apiculata ethanolic extract through Gas Chromatography-Mass Spectrometry (GC-MS) analysis and Fourier-Transform Infrared (FTIR) spectral analysis. Qualitative phytochemical screening results revealed the presence of alkaloids, saponins, phenols, glycosides, steroids, and tannins. GC-MS analysis identified a total of 12 compounds, encompassing antioxidants, fatty acids, esters, and steroidal ketones, while FTIR affirmed the presence of functional groups (-OH, -NH2, C = O and -COOH) associated with antibacterial activity. The extract displayed significant antibacterial effects against oral pathogens, showing the highest activity against K. pneumoniae. The biosafety of the extract was assessed by exposing it to adult zebrafish larvae, and no acute toxicity was exhibited, suggesting it is safe for instant use. Overall, this report underscores R. apiculata crude extract to be a promising antibacterial agent against K. pneumoniae.
