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[Fluorescent Probe Development for Rapid Detection of Tiletamine Based on Copper Nanozyme and Molecular Imprinting
Jia-Hao Li1, Jiang Ling1, Zi-Hao Cai1
1Department of Forensic Medicine, Xiangya School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Objectives:
To develop a rapid detection method for tiletamine that is easy to operate and low-cost under the premise of ensuring sensitivity and accuracy, to assist in carrying out rapid screening and drug control work on-site.
Methods:
This study integrates dual-ligand copper nanozymes with molecular imprinting technology. Initially, copper nanozymes were synthesized using readily available raw materials at 120 ℃. Subsequently, specific cavities were imprinted on their surface at room temperature using a sol-gel method to construct a novel fluorescent sensing probe. This probe was characterized and methodologically validated, and then applied to the detection of actual samples.
Results:
The developed probe exhibited stable fluorescence properties, strong anti-interference capability, and excellent specificity and sensitivity, with a detection limit of 5 ng/mL and a quantitative concentration range from 15 to 500 ng/mL. It enabled the rapid detection of tiletamine in real samples such as blood and e-cigarette oil.
Conclusions:
This fluorescent probe can be used for rapid detection and on-site preliminary screening of tiletamine in various types of samples. It significantly improves the detection efficiency and reduces analysis costs, showing high research value and broad application prospects.
