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Updated: Jan 8, 2026

Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon
Published on: October 29, 2021
Phosphorylated Salmon bone polypeptides: enhancing stability and antimicrobial activity through zinc chelation
Lingyu Han1, Nuo Dong1, Bing Hu1
1Key Lab of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian, Liaoning 116600, China.
None:
Salmon bones, a marine waste, have bioactive potential. Salmon bone peptides (SBPs) were phosphorylated at different pH to form P-SBPs, whose structure and that of their zinc chelates (P-SBPs-Zn) were analyzed via Fourier-transform infrared spectroscopy, fluorescence spectroscopy and CD spectrum. Among the pH-modified variants, phosphopeptide‑zinc chelates at pH 4 (P-SBPs4-Zn) exhibited the best solubility and highest zinc chelating property, with a zinc content of 86.15 ± 0.47 mg/g. The stability and digestibility were tested under simulated gastrointestinal conditions (adults/elderly, dietary components). P-SBPs4-Zn demonstrated optimal stability and digestibility. After evaluation the antibacterial activity, P-SBPs4-Zn exhibited significant antibacterial effects against Escherichia coli and Staphylococcus aureus through mechanisms involving membrane disruption. The safety assessment using Caco-2 and RAW 264.7 cells confirmed its biocompatibility, supporting its suitability for oral application. This supports its use as an efficient, stable, antibacterial zinc supplement for food processing.
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