Related Experiment Video
Updated: Jan 7, 2026
08:51
Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Published on: June 25, 2015
9.6K
DUSP1 interacts with BIP to regulate Staphylococcus aureus-induced apoptosis through the MAPK signaling pathway
Xuyang Zhang1, Zhanyou Liu1, Guilan Ma1
1School of Life Sciences, Ningxia University, Yinchuan, Ningxia 750021, China; Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, Ningxia 750021, China.
Microbiological Research
|December 25, 2025
Summary
Staphylococcus aureus infection triggers apoptosis by increasing Dual-specificity phosphatase-1 (DUSP1) and BIP expression. DUSP1 interacts with BIP to regulate apoptosis via the MAPK pathway, offering a potential therapeutic target.
Area of Science:
- Immunology
- Cell Biology
- Microbiology
Background:
- Staphylococcus aureus (S. aureus) is a pathogen causing immune cell apoptosis, impacting disease severity.
- Dual-specificity phosphatase-1 (DUSP1) regulates MAPK signaling but its role in S. aureus-induced apoptosis is unknown.
Purpose of the Study:
- To investigate the function and regulatory mechanisms of DUSP1 in S. aureus-induced apoptosis.
Main Methods:
- Studied S. aureus infection models.
- Analyzed DUSP1 and BIP expression levels.
- Performed DUSP1 knockdown and BIP inhibition experiments.
- Assessed apoptosis, reactive oxygen species, and MAPK signaling.
Main Results:
- S. aureus infection induced DUSP1 and BIP expression, promoting apoptosis.
- DUSP1 knockdown exacerbated apoptosis, ROS accumulation, and MAPK activation.
- DUSP1 binds to BIP, and BIP inhibition enhanced apoptosis and MAPK signaling.
Conclusions:
- S. aureus infection induces DUSP1 and BIP, leading to apoptosis via the MAPK pathway.
- DUSP1 interacts with BIP to regulate S. aureus-induced apoptosis.
- DUSP1 represents a potential therapeutic target for S. aureus infections.
More Related Videos
Related Concept Videos
01:31The Intrinsic Apoptotic Pathway
8.2K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.2K
01:07MAPK Signaling Cascades
7.8K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.8K
01:19Interactions Between Signaling Pathways
7.1K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.1K
01:20The JAK-STAT Signaling Pathway
11.8K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
11.8K
01:17The Extrinsic Apoptotic Pathway
8.0K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
8.0K
01:24Caspases
13.6K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
13.6K