Rational Design of Light-Responsive Nanozyme with Reversible Inhibitory Function for Colorimetric Sensing of
Yuting Jiang1, Liuding Wang1, Yuanlin Huang1
1Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Guangxi Key Laboratory of Chemistry and Molecular Engineering of Medicinal Resources, University Engineering Research Center for Chemistry of Characteristic Medicinal Resources (Guangxi), School of Chemistry and Pharmaceutical Science, Guangxi Normal University, Guilin 541004, P. R. China.
Analytical Chemistry
|December 26, 2025
Summary
This study reveals reversible inhibition mechanisms in nonmetallic nanozymes using a light-responsive material. A novel colorimetric platform was developed for sensitive detection of diclofenac sodium.
Area of Science:
- Nanomaterials Science
- Biochemistry
- Analytical Chemistry
Background:
- Studying reversible nanozyme inhibition is challenging due to irreversible adsorption in metal-based materials.
- Nonmetallic porous materials offer advantages for reversible inhibition studies due to their nature and adsorption properties.
Purpose of the Study:
- To investigate the reversible inhibition mechanisms of a nonmetallic porous light-responsive nanozyme (TTBT·Cl) using diclofenac sodium (DS) as a model inhibitor.
- To develop a sensitive colorimetric platform for DS detection.
Main Methods:
- Utilized a nonmetallic porous light-responsive nanozyme (TTBT·Cl) and diclofenac sodium (DS).
- Employed comprehensive characterization and experimental analyses to elucidate inhibition mechanisms.
- Developed a selective and sensitive colorimetric platform for DS detection.
Main Results:
- TTBT·Cl demonstrated high adsorption capacity for oxygen substrates and facilitated mass transfer.
- The nanozyme exhibited light-responsive oxidase-like activity.
- Photocatalytic and uncompetitive inhibition mechanisms were clarified.
Conclusions:
- The study clarifies reversible inhibition mechanisms in nonmetallic nanozymes.
- A novel colorimetric platform enables selective and sensitive DS detection.
- Provides a framework for studying other nanozyme systems and biosensing applications.
