Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Triphase Interfacial Nucleation Enables Microneedle-Templated Metal-Organic Framework Coating for <i>In Situ</i> DNA Capture and Release.

Analytical chemistry·2026

Same author

A Near-Infrared-Triggered Luminescence-Activated System for In Vivo Biomacromolecular Tagging and Photocatalytic Crosslinking for Large-Scale Investigation of RNA-Protein Complexes in Living Mice.

Journal of the American Chemical Society·2026

Same author

Octadecylamine-Modified CuO NPs Enabling Highly Selective <i>In Vivo</i> Ascorbic Acid Potentiometric Detection with Enhanced Sulfide Tolerance.

ACS sensors·2026

Same author

Effect of insoles with different cushioning properties on knee impact after radial tear of posterior horn of medial meniscus: a finite element analysis.

BMC musculoskeletal disorders·2026

Same author

Simulated microgravity-driven mechanical unloading rescues PIEZO1-overexpression-induced growth plate ossification and retards adolescent idiopathic scoliosis.

NPJ microgravity·2026

Same author

Distribution network risk prediction based on data mining and improved PSO fused with SVM.

Scientific reports·2026

Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026

Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026

Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026

Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026

Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026

Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 7, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Hierarchically Engineered Phage-Activated Click Interface for Ultra-Efficient Cell Capture.

Huida Li¹, Rui Wang¹, Fengting Jia¹

¹Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.

Analytical Chemistry

|December 27, 2025

Summary

This summary is machine-generated.

This study introduces a novel multiscale cell capture interface (PACE-Chip) that significantly enhances cell binding strength and kinetics. The engineered chip efficiently isolates target cells from complex samples, outperforming traditional 2D methods.

More Related Videos

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy

Published on: October 19, 2018

Related Experiment Videos

Last Updated: Jan 7, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy

Published on: October 19, 2018

Area of Science:

Biomaterials Engineering
Cell Biology
Microfluidics

Background:

Efficient cell adhesion under flow is crucial for biological processes and artificial cell capture systems.
Existing methods primarily focus on molecular-level enhancements in 2D, neglecting multiscale strategies.
Simultaneous enhancement of cell-surface encounter frequency and binding probability across spatial scales is underexplored.

Purpose of the Study:

To develop a multiscale, hierarchically engineered cell capture interface for synergistic enhancement of cell binding.
To investigate the impact of integrating molecular, microscale, and macroscale features on cell capture efficiency.
To optimize phage scaffold length for improved cell capture performance.

Main Methods:

Engineered a multiscale hierarchically interface, PACE-Chip, integrating click chemistry, M13 phage scaffolds, and herringbone structures.
Evaluated binding strength and kinetics of the PACE-Chip against a 2D counterpart.
Assessed the efficiency of target cell isolation from complex blood matrices.
Analyzed the effect of phage length on binding performance.

Main Results:

The PACE-Chip demonstrated a 294% increase in binding strength and a 181-fold acceleration in binding kinetics.
Achieved highly efficient isolation of target cells down to the single-cell level from complex blood matrices.
Identified that excessive phage length negatively impacts performance due to entropic and structural penalties.

Conclusions:

The multiscale hierarchical engineering approach synergistically enhances cell binding kinetics and affinity.
PACE-Chip offers a superior platform for efficient cell isolation compared to traditional 2D interfaces.
A multiscale trade-off exists between multivalency and conformational stability, necessitating optimization of phage scaffold length.