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Updated: Jan 7, 2026

Extraction of Plant-based Capsules for Microencapsulation Applications
Published on: November 9, 2016
Encapsulation of vitexin using calcium carbonate microparticles enhances stability, release, and anti-inflammatory
Methavee Peanparkdee1, Kanokrat Limpisophon1, Pakamon Chitprasert2
1Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, 50 Ngamwongwan Road Ladyao Chatuchak Bangkok, 10900, Thailand.
Abstract:
Vitexin, a C-glycosylated flavonoid with poor aqueous solubility and low bioaccessibility, was incorporated into a plant-based protein-rich matrix in free and encapsulated forms to evaluate its stability, release, and bioactivity. Calcium carbonate microparticles were used as encapsulation carriers. Formulations containing free vitexin, encapsulated vitexin, or wall material were assessed for their physical properties, phenolic profile changes during simulated digestion, and anti-inflammatory activity in LPS-induced HepG2 cells. Both free and encapsulated vitexin improved water retention and cohesiveness without significantly affecting shear force. Free vitexin increased yellowness and browning index. UHPLC-MS/MS analysis revealed changes in phenolic composition during digestion, with encapsulation significantly enhancing vitexin stability. The encapsulated vitexin formulation reduced intracellular reactive oxygen species and downregulated IL6, TNFα, and IL1β gene expression more effectively than free vitexin. These findings highlight the potential of calcium carbonate microparticle encapsulation to improve the digestive stability and functional efficacy of vitexin in complex food matrices.
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