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Published on: June 28, 2024
A self-fluorescent PS-NH2-BDC@Fe2O3 nanozyme-enabled dual-mode biosensing platform for ultrasensitive quantification
Mengting Chen1, Tongyue Yin2, Yuming Yang1
1The National Administration of Traditional Chinese Medicine's Tertiary Laboratory for Pharmacology of Traditional Chinese Medicine (Oncology) Research, College of Medicine and Health Sciences, China Three Gorges University, Yichang 443002, China.
Abstract:
Developing efficient and reliable sensing strategies for the detection of ochratoxin A (OTA) in complex food matrices is crucial to prevent mycotoxin-related diseases. Herein, a dual-mode immunosensing platform based on self-fluorescent nanozyme was successfully constructed for the ultrasensitive detection of OTA. A porous spherical Fe2O3 nanozyme modified with an NH2-BDC ligand (PS-NH2-BDC@Fe2O3) was synthesized via a one-step hydrothermal method. This material not only exhibited high peroxidase (POD)-like activity due to its unique mesoporous structure and Fe2+/Fe3+ redox cycle, but also possessed self-fluorescent properties, thereby avoiding the introduction of exogenous fluorophores. Following a competitive immunoassay, the platform induced localized surface plasmon resonance (LSPR)-mediated multicolor changed in gold nanorods (Au NRs) via the H2O2/TMB reaction, while incorporating a ratiometric fluorescence system constructed from bovine serum albumin-templated gold nanoclusters (BSA-Au NCs) and PS-NH2-BDC@Fe2O3-Ab2 for dual-mode OTA detection. The developed immunosensor exhibited a wide linear range from 0.001 to 10 μg/L with a low detection limit of 2.2∗10-4 μg/L. By combining intuitive multicolor visualization with self-calibrated dual-signal output, this work provides a novel and biocompatible sensing strategy for detecting trace mycotoxins in food.
